教育经历:
2000-2006年，博士，神经生物学系，杜克大学
1996-2000年，学士，新葡亰8814网站是多少，北京大学
工作经历:
2020-今，教授，新葡亰8814网站是多少，北京大学
2012-今，研究员，新葡亰8814网站是多少，生命科学联合中心，麦戈文脑研究所，北京大学
2019-2020年，副教授，新葡亰8814网站是多少，北京大学
2012-2019年，助理教授，新葡亰8814网站是多少，北京大学
2006-2012年，博士后，分子和细胞生理学系，斯坦福大学荣誉奖励:
科学探索奖生命科学奖，2019
国家杰出青年科学基金，2019
第十二届谈家桢生命科学创新奖，2019
张香桐神经科学青年科学家奖，2019
中国十大医学科技新闻，2018
中国生命科学十大进展，中国科协生命科学学会联合体，2018
中源协和生命医学奖创新突破奖，2018
勃林格殷格翰研究员奖，2018
绿叶生物医药杰出青年学者奖，2015
学术任职:
2019-至今，Journal of Neurochemistry，编委
2018-至今，美洲华人生物科学学会，会员
2001-至今，美国神经科学学会，成员执教课程:
遗传学讨论课
整合科学实验课
生命科学强化挑战班文献讨论课
cls神经生物学track
ptn神经生物学track
高级神经生物学 人的大脑由数十亿的神经元组成，后者又通过数万亿的突触组成复杂的神经网络。不同种类的神经元经过或远或近的投射，通过突触与其他神经元进行信息交流，实现感知觉、决策和运动等高级神经功能。
研究大脑的最大挑战在于脑的高度复杂性。我们实验室集中在神经元通讯的基本结构突触上，从两个层面上开展研究：一是开发前沿的工具，即开发新型成像探针，用于在时间和空间尺度上解析神经系统的复杂功能；二是借助先进的工具探究突触传递的调节机制，特别是在生理及病理条件下对神经递质释放的调节。
具体而言，对于工具开发，我们集中于：
1，结合光遗传学和荧光成像，无损伤性的研究神经元之间的电突触连接。电突触的异常可导致耳聋、癫痫、脑部肿瘤和心脏功能异常等疾病。
2，开发可遗传编码的检测神经递质/调质的荧光探针。神经递质/调质是神经元化学突触传递的关键介导分子，与感知、学习和记忆以及情绪密切相关。
利用上述荧光探针，我们的功能性和生理性的研究集中于：
1，结合双光子成像和可遗传编码的荧光探针，使用果蝇和小鼠作为模式生物，研究嗅觉传导或睡眠过程中脑的工作机制。
2，寻找上述新型化学递质/调质小分子的对应受体，即寻找“孤儿”受体的配体。
3，结合生物信息学、分析化学、生物化学、生理学和成像学方法，系统地探索和鉴定潜在的新型小分子神经递质。
Main Research Articles
· Sun, F.#, Zhou, J.#, Dai, B.#, Qian, T., Zeng, J., Li, X., Zhuo, Y., Zhang, Y., Wang, Y., Qian, C., Tan, K., Feng, J., Dong, H., Lin, D.*, Cui, G.*, & Li, Y.*.(2020). Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Nature Methods, https://doi.org/10.1038/s41592-020-00981-9.
· Jing, M.*, Li, Y., Zeng, J., Huang, P., Skirzewski, M., Kljakic, O., Peng, W., Qian, T., Tan, K., Wu, R., Zhang, S., Pan, S., Xu, M., Li, H., Saksida, L. M., Prado, V. F., Bussey, T., Prado, M. A. M., Chen, L., Cheng, H., Li, Y.*.(2020). An optimized acetylcholine sensor for monitoring in vivo cholinergic activity. Nature Methods, https://doi.org/10.1038/s41592-020-0953-2.
· Yu, H., Zhao, T., Liu, S., Wu, Q., Johnson, O., Wu, Z., Zhuang, Z., Shi, Y., He, R., Yang, Y., Sun, J., Wang, X., Xu, H., Zeng, Z., Lei, X., Luo, W.* & Li, Y.*. (2019). MRGPRX4 is a bile acid receptor for human cholestatic itch. eLife, 8, e48431.
· Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J. & Li, Y.* (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine. Neuron, 102(4), 745-761.
· Wu, Z.#, Feng, J.#, Jing, M., & Li, Y.* (2019). G protein-assisted optimization of GPCR-activation based (GRAB) sensors. Neural Imaging and Sensing 2019, vol. 10865, p. 108650N. International Society for Optics and Photonics.
· Wu, L., Dong, A., Dong, L., Wang, S. Q., & Li, Y*. (2019). PARIS, an optogenetic method for functionally mapping gap junctions. eLife, 8, e43366.
· Sun, F.#, Zeng, J.#, Jing, M.#, Zhou, J., Feng, J., Owen, S., Luo, Y., Li, F., Wang, H., Yamaguchi, T., Yong, Z., Gao, Y., Peng, W., Wang, L., Zhang, S., Du, J., Lin, D., Xu, M., Kreitzer, A. C., Cui, G. & Li, Y.* (2018). A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice. Cell, 174(2), 481-496.
· Jing, M.#, Zhang, P.#, Wang, G., Feng, J., Mesik, L., Zeng, J., Jiang, H., Wang, S., Looby, J. C., Guagliardo, N. A., Langma, L. W., Lu, J., Zuo, Y., Talmage, D. A., Role, L. W., Barrett, P. Q., Zhang, L. I., Luo, M., Song, Y., Zhu, JJ* & Li, Y*. (2018). A genetically-encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Nature Biotechnology, 36(8), 726-737.
· Li, Y.*, & Tsien, R. W.* (2012). pHTomato, a red, genetically encoded indicator that enables multiplex interrogation of synaptic activity. Nature neuroscience, 15(7), 1047-1053.
· Li, Y., Augustine, G. J., & Weninger, K.* (2007). Kinetics of complexin binding to the SNARE complex: correcting single molecule FRET measurements for hidden events. Biophysical journal, 93(6), 2178-2187.
Collaborative Publications
· Wang, J.#, Li, J.#, Yang, Q.#, Xie, Y.-K., Wen, Y.-L., Xu, Z.-Z., Li, Y., Xu, T., Wu, Z.-Y., Duan, S., & Xu, H.* (2021). Basal forebrain mediates prosocial behavior via disinhibition of midbrain dopamine neurons. Proceedings of the National Academy of Sciences，118(7), e2019295118. https://doi.org/10.1073/pnas.2019295118。
· Song, Y., Xu, C., Liu, J., Li, Y., Wang, H., Shan, D., Wainer Irving, W., Hu, X., Zhang, Y.*, Woo Anthony, Y.-H.*, & Xiao, R.-P. Heterodimerization with 5-HT2BR Is Indispensable for β2AR-mediated Cardioprotection. Circulation Research, https://doi.org/10.1161/CIRCRESAHA.120.317011。
· Zhu, R.#, Zhang, G.#, Jing, M., Han, Y., Li, J., Zhao, J., Li, Y., & Chen, P. R.* (2021, 2021/01/25). Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction. Nature Communications,，12(1), 581. https://doi.org/10.1038/s41467-020-20754-4。
· Mayer, F. P., Iwamoto, H., Hahn, M. K., Grumbar, G. J., Stewart, A., Li, Y., & Blakely, R. D.* (2021). There`s no place like home? Return to the home cage triggers dopamine release in the mouse nucleus accumbens. Neurochemistry International, 142, 104894. https://doi.org/https://doi.org/10.1016/j.neuint.2020.104894
· Bari*, A., Xu, S., Pignatelli, M., Takeuchi, D., Feng, J., Li, Y., & Tonegawa, S.* (2020). Differential attentional control mechanisms by two distinct noradrenergic coeruleo-frontal cortical pathways. Proceedings of the National Academy of Sciences, https://doi.org/10.1073/pnas.2015635117。
· Kim, H. R.*, Malik, A. N., Mikhael, J. G., Bech, P., Tsutsui-Kimura, I., Sun, F., Zhang, Y., Li, Y., Watabe-Uchida, M., Gershman, S. J., & Uchida, N.* (2020). A Unified Framework for Dopamine Signals across Timescales. Cell, https://doi.org/https://doi.org/10.1016/j.cell.2020.11.013。
· Crouse,R. B., Kim, K., Batchelor, H. M., Kamaletdinova, R., Chan, J., Rajebhosale, P., Pittenger, S. T., Role, L. W., Talmage, D A., Jing, M. , Li, Y., Gao, X., Mineur , Y. S., & Picciotto, M. R. * (2020). Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances learning of cue-reward contingency. eLife, 9:e57335.
· Kwak, H., Koh, W., Kim, S., Song, K., Shin, J., Lee, J. M., Lee, E. H., Bae, J. Y., Ha, G. E., Oh, J. Park, Y. M., Kim, S., Feng, J., Lee, S. E., Choi, J. W., Kim, K. H., Kim, Y. S., Woo, J., Lee, D., Son, T., Kwon, S. W., Park, K. D., Yoon, B. Lee, J., Li, Y. , Lee, H., Bae, Y. C., Lee, C. J.* & Cheong, E.* (2020). Astrocytes Control Sensory Acuity via Tonic Inhibition in the Thalamus. Neuron, https://doi.org/10.1016/j.neuron.2020.08.013.
·Peng, W.#, Wu, Z.#, Kun, S.#, Zhang, S., Li, Y. & Min, X.* (2020). Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons. Science, 369, 1208.
· Mazzone, C.M., Liang-Guallpa, J.,Li, C., Wolcott, N. S., Boone, M. H., Southern, M., Kobzar, N. P., Salgado, I. A., Reddy, D. M., Sun, F., Zhang, Y., Li, Y., Cui, G. * & Krashes, M. J.* (2020). High-fat food biases hypothalamic and mesolimbic expression of consummatory drives. Nature Neuroscience, https://doi.org/10.1038/s41593-020-0684-9.
· DeGroot, S.R., Zhao-Shea, R., Chung L., Klenowski, P.M., Sun, F., Molas, S., Gardner, P.D., Li, Y. & Tapper, A.R.* (2020). Midbrain dopamine controls anxiety-like behavior by engaging unique interpeduncular nucleus microcircuitry. Biological Psychiatry, https://doi.org/10.1016/j.biopsych.2020.06.018.
· Zhu, P. K. ,Zheng, W. S. , Zhang, P., Jing, M., Borden, P. M., Ali, F., Guo, K., Feng, J., Marvin, J. S., Wang, Y., Wan, J., Gan, L., Kwan, A. C., Lin, L., Looger, L. L., Li, Y. & Zhang, Y.* (2020). Nanoscopic visualization of restricted nonvolume cholinergic and monoaminergic transmission with genetically encoded sensors. Nano Lett., https://doi.org/10.1021/acs.nanolett.9b04877.
· Lin, R., Liang, J., Wang, R., Yan, T., Zhou, Y., Liu, Y., Feng, Q., Sun, F.,, Li, Y., Li, A., Gong, H., & Luo, M.* (2020). The Raphe Dopamine System Controls the Expression of Incentive Memory. Neuron, 1420-19.
· Zhang, X., Noyes, N. C. , Zeng, J., Li, Y. & Davis, R. L.* (2019). Aversive Training Induces Both Pre- and Postsynaptic Suppression in Drosophila. The Journal of Neuroscience, 1420-19.
· Handler, A., Graham, T. G. M., Cohn, R., Morantte, I., Siliciano, A. F., Zeng J., Li, Y. & Ruta, V.* (2019). Distinct dopamine receptor pathways underlie the temporal sensitivity of associative learning. Cell, 178(1), 60-75.
· Liang, X., Ho, M. C., Zhang, Y., Li, Y., Wu, M. N., Holy, T. E., & Taghert, P. H.* (2019). Morning and Evening Circadian Pacemakers Independently Drive Premotor Centers via a Specific Dopamine Relay. Neuron, 102(4), 843-857.
· Zhou, M.#, Chen, N.#, Tian, J., Zeng, J., Zhang, Y., Zhang, X., Guo, J., Sun, J., Li, Yulong., Guo, A.*, & Li, Yan.* (2019). Suppression of GABAergic neurons through D2-like receptor secures efficient conditioning in Drosophila aversive olfactory learning. PNAS, 201812342.
· Li, B.#, Wong, C.#, Gao, S. M., Zhang, R., Sun, R., Li, Y., & *Song, Y. (2018). The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking. eLife, 7, e38181.
· Tanaka, M., Sun, F., Li, Y., & Mooney, R.* (2018). A mesocortical dopamine circuit enables the cultural transmission of vocal behaviour. Nature, 563(7729), 117-120.
· Chen, B.#, Huang, X.#, Gou, D., Zeng, J., Chen , G., Pang, M., Hu, Y., Zhao, Z., Wu, H., Cheng, H., Zhang, Z., Xu, C., & Li, Y., Chen, L.*, Wang, A.* (2018). Rapid volumetric imaging with Bessel-Beam three-photon microscopy. Biomedical optics express, 9(4), 1992-2000.
· Shen, Y., Ge, W. P., Li, Y., Hirano, A., Lee, H. Y., Rohlmann, A., Missler, M., Tsien, R. W., Jan, L. Y., Fu, Y. H.* & Ptacek, L. J.* (2015). Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis. Proceedings of the National Academy of Sciences, 112(10), 2935-2941.
· Liang, L., Li, Y., Potter, C. J., Yizhar, O., Deisseroth, K., Tsien, R. W., & Luo, L.* (2013). GABAergic Projection Neurons Route Selective Olfactory Inputs to Specific Higher-Order Neurons. Neuron, 79(5), 917-931.
· Park, H., Li, Y., & Tsien, R. W.* (2012). Influence of synaptic vesicle position on release probability and exocytotic fusion mode. Science, 335(6074), 1362-1366.
· Yoo, A. S.*, Sun, A. X., Li, L., Shcheglovitov, A., Portmann, T., Li, Y., Lee-Messer, C., Dolmetsch, R. E., Tsien R. W. & Crabtree, G. R.* (2011). MicroRNA-mediated conversion of human fibroblasts to neurons. Nature, 476(7359), 228-231.
· Zhang, Q., Li, Y., & Tsien, R. W.* (2009). The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles. Science, 323(5920), 1448-1453.
· Kuner, T.*, Li, Y., Gee, K. R., Bonewald, L. F., & Augustine, G. J. (2008). Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. Proceedings of the National Academy of Sciences, 105(1), 347-352.
Reviews, Book Reviews and Highlights
· Wan, J. & Li, Y.* (2020). Recent Advances in Detection Methods for Neurotransmitters. Chinese Journal of Analytical Chemistry, 48(3), 307-315. (In Chinese)
· Wu, Z.* & Li, Y.* (2020). New frontiers in probing the dynamics of purinergic transmitters in vivo. Neuroscience Research, https://doi.org/10.1016/j.neures.2020.01.008.
· Zeng, J., Sun, F., Wan, J., Feng, J. & Li, Y.* (2019). New optical methods for detecting monoamine neuromodulators. Current Opinion in Biomedical Engineering, https://doi.org/10.1016/j.cobme.2019.09.010.
· Jing, M., Zhang, Y., Wang, H. & Li, Y.* (2019). GPCR‐based sensors for imaging neurochemicals with high sensitivity and specificity. Journal of Neurochemistry, https://doi.org/10.1111/jnc.14855.
· Dong, A.*, Liu, S., & Li, Y.* (2018). Gap Junctions in the Nervous System: Probing Functional Connections Using New Imaging Approaches. Frontiers in Cellular Neuroscience, 12, 320.
· Wang, H., Jing, M., & Li, Y.* (2018). Lighting up the brain: genetically encoded fluorescent sensors for imaging neurotransmitters and neuromodulators. Current Opinion in Neurobiology, 50, 171-178.
· Wang, A.#, Feng, J.#, Li, Y.*, & Zou, P.* (2018). Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities. ACS chemical neuroscience, 9(4), 639-650.
· Qian, C., & Li, Y.* (2015). Spine maturation and pruning during development: Cadherin/Catenin complexes come to help. Science China. Life sciences,58(9), 929.
· Li, Y.*, & Rao, Y.* (2015). Pied Piper of Neuroscience. Cell, 163(2), 267-268.
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